BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Mechanobiology Institute - ECPv6.15.17//NONSGML v1.0//EN CALSCALE:GREGORIAN METHOD:PUBLISH X-WR-CALNAME:Mechanobiology Institute X-ORIGINAL-URL:https://mbi.closelycoded.site X-WR-CALDESC:Events for Mechanobiology Institute REFRESH-INTERVAL;VALUE=DURATION:PT1H X-Robots-Tag:noindex X-PUBLISHED-TTL:PT1H BEGIN:VTIMEZONE TZID:Asia/Shanghai BEGIN:STANDARD TZOFFSETFROM:+0800 TZOFFSETTO:+0800 TZNAME:CST DTSTART:20250101T000000 END:STANDARD END:VTIMEZONE BEGIN:VTIMEZONE TZID:UTC BEGIN:STANDARD TZOFFSETFROM:+0000 TZOFFSETTO:+0000 TZNAME:UTC DTSTART:20200101T000000 END:STANDARD END:VTIMEZONE BEGIN:VEVENT DTSTART;TZID=Asia/Shanghai:20260325T150000 DTEND;TZID=Asia/Shanghai:20260325T170000 DTSTAMP:20260605T182857 CREATED:20260313T023117Z LAST-MODIFIED:20260313T023226Z UID:10000530-1774450800-1774458000@mbi.closelycoded.site SUMMARY:SPSS DESCRIPTION:First Speaker: \n\n\n\n \n\n\n\nSecond Speaker: URL:https://mbi.closelycoded.site/event/spss/2026-03-25/ LOCATION:T-Lab Level 5 Seminar Room CATEGORIES:SPSS END:VEVENT BEGIN:VEVENT DTSTART;TZID=Asia/Shanghai:20260318T150000 DTEND;TZID=Asia/Shanghai:20260318T170000 DTSTAMP:20260605T182857 CREATED:20260313T023117Z LAST-MODIFIED:20260313T023226Z UID:10000529-1773846000-1773853200@mbi.closelycoded.site SUMMARY:SPSS DESCRIPTION:First Speaker: \n\n\n\n \n\n\n\nSecond Speaker: URL:https://mbi.closelycoded.site/event/spss/2026-03-18/ LOCATION:T-Lab Level 5 Seminar Room CATEGORIES:SPSS END:VEVENT BEGIN:VEVENT DTSTART;TZID=UTC:20260312T100000 DTEND;TZID=UTC:20260312T110000 DTSTAMP:20260605T182857 CREATED:20260313T024414Z LAST-MODIFIED:20260520T020242Z UID:10000791-1773309600-1773313200@mbi.closelycoded.site SUMMARY:PhD Qualifying Exam DESCRIPTION:Examinee:\nKottpalli VIDHIPRIYA\, Young Group\n \nTitle: \nELUCIDATING THE AGE AND STIFFNESS REGULATION OF THE TISSUE RESIDENT MACROPHAGE POLARIZATION\n \nAbstract:\nAging and cardiac fibrosis are associated with increased tissue stiffness and chronic inflammation\, which are critical drivers of cardiac remodelling and contribute to the development of heart failure. Importantly\, cardiac tissue-resident (TR) macrophages are among the most abundant immune cell types and are specialized cells responsible for maintaining tissue homeostasis\, immune surveillance\, and regulation of inflammatory response. However\, the mechanism by which age-associated changes in ECM mechanical properties regulate cardiac TR macrophage polarisation remains poorly understood. We hypothesize that increased tissue stiffness during aging drives TR macrophages towards a pro-inflammatory-like phenotype mediated by mechanosensitive genes. Here\, our reanalysis of a publicly available SMART-Seq transcriptomics dataset of young and aged murine cardiac macrophages (Xia et al.\, 2024) revealed that TR macrophages are transcriptionally distinct from monocyte-derived (M-d) macrophages\, with ~87% of age- associated differentially expressed genes are lineage specific\, downregulation of ECM organization and actomyosin pathways and identification of 18 TR specific mechanosensitive genes dysregulated with aging. To experimentally test this mechanism\, we isolated cardiac TR macrophages using CD45+ magnetic bead enrichment and confirmed purity by immunofluorescence staining with CD68 and LYVE1 markers\, as well as by fluorescence-activated cell sorting (FACS) using CD45\, CD68\, and CCR2 markers\, achieving ~80% CCR2- TR macrophage purity. These isolated cells were then cultured on polyacrylamide hydrogels with stiffness mimicking young (10 kPa) and aged (40 kPa) cardiac tissue stiffness\, suggesting a stiffness-dependent shift towards a pro-inflammatory-like phenotype assessed by iNOS/CD206 fluorescence intensity ratio (n=1\, N=3 technical replicates). To further define the mechanism\, we will investigate the identified mechanosensitive signaling pathways mediating stiffness-dependent TR macrophage polarisation. Furthermore\, we have fabricated a polyacrylamide stiffness gradient hydrogel platform (E=12 to 60 kPa)\, which will be functionalised with decellularized ECM from young and aged murine cardiac tissue to recapitulate the cardiac microenvironment. This approach will enable us to define the stiffness-dependent transition from anti- to pro-inflammatory phenotype in cardiac TR macrophages. Overall\, this project aims to provide mechanistic insights into how age-associated tissue stiffness drives chronic inflammation and will contribute to our understanding of immune-mechanical interactions in age-associated cardiac remodelling.\n\nKey words: Cardiac aging\, Resident macrophages\, Stiffness gradient\, PA hydrogels. \n\n \nImportant Note\nThe examination following the seminar is a closed-door session. All attendees are requested to exit from the meeting room after student’s presentation/Q&A. URL:https://mbi.closelycoded.site/event/phd-qualifying-exam-2/ LOCATION:T-Lab Level 5 Seminar Room CATEGORIES:PhD Qualifying Exam ATTACH;FMTTYPE=image/jpeg:https://mbi.closelycoded.site/wp-content/uploads/2026/03/phd_square.jpg END:VEVENT BEGIN:VEVENT DTSTART;TZID=UTC:20251128T080000 DTEND;TZID=UTC:20251130T170000 DTSTAMP:20260605T182857 CREATED:20251128T071547Z LAST-MODIFIED:20251202T073954Z UID:10000003-1764316800-1764522000@mbi.closelycoded.site SUMMARY:Event Testing Hura Hura DESCRIPTION:« All Events\n \nThis event has passed. \n\n Event Testing Hura Hura\nOverview\n https://www.youtube.com/watch?v=XHOmBV4js_E\n November 28\n @\n8:00 am\n –\n November 30\n @\n5:00 pm \n\n \n Harpy \n \n\nHappiness Street\n \n Singapore\,\n Singapore\n Singapore\n\n\n + Google Map \n\n \n \n \n\n\n \nSpeakers\nExploring Other Events URL:https://mbi.closelycoded.site/event/event-testing-hura-hura/ LOCATION:Harpy\, Happiness Street\, Singapore\, Singapore\, Singapore END:VEVENT BEGIN:VEVENT DTSTART;TZID=UTC:20251029T080000 DTEND;TZID=UTC:20251029T170000 DTSTAMP:20260605T182857 CREATED:20251014T051240Z LAST-MODIFIED:20251014T051240Z UID:10000002-1761724800-1761757200@mbi.closelycoded.site SUMMARY:Testing Event #2 DESCRIPTION:Lorem ipsum dolor sit amet\, consectetur adipiscing elit\, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam\, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident\, sunt in culpa qui officia deserunt mollit anim id est laborum. URL:https://mbi.closelycoded.site/event/testing-event-2/ LOCATION:Harpy\, Happiness Street\, Singapore\, Singapore\, Singapore ATTACH;FMTTYPE=image/png:https://mbi.closelycoded.site/wp-content/uploads/2025/07/893cfb8e63b8298df23fd502614ba7d7c26cba4a.png END:VEVENT BEGIN:VEVENT DTSTART;TZID=UTC:20251024T080000 DTEND;TZID=UTC:20251024T170000 DTSTAMP:20260605T182857 CREATED:20251014T051119Z LAST-MODIFIED:20251014T051119Z UID:10000001-1761292800-1761325200@mbi.closelycoded.site SUMMARY:Testing Event #1 DESCRIPTION:Lorem ipsum dolor sit amet\, consectetur adipiscing elit\, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam\, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident\, sunt in culpa qui officia deserunt mollit anim id est laborum. URL:https://mbi.closelycoded.site/event/testing-event-1/ LOCATION:Harpy\, Happiness Street\, Singapore\, Singapore\, Singapore END:VEVENT BEGIN:VEVENT DTSTART;TZID=UTC:20210729T080000 DTEND;TZID=UTC:20210807T170000 DTSTAMP:20260605T182857 CREATED:20251202T083835Z LAST-MODIFIED:20251202T111222Z UID:10000004-1627545600-1628355600@mbi.closelycoded.site SUMMARY:Bootcamp 2021 DESCRIPTION:« All Events\n \n\n Bootcamp 2021\n July 29\, 2021\n –\n August 7\, 2021\n \n About\n \n \n People\n \n \n Program\n \n \n Registration and Location\n \n \n Sponsor\n \nThis ten-day “Bootcamp” style course will cover fundamental concepts and practical approaches in understanding cellular functions. The focus is to develop a breadth of knowledge in quantitative techniques to allow bioscience researchers to pursue further depth in their respective research work. \nThe Bootcamp lectures are open to all interested graduate students and post-docs. For graduate students the lectures will be reinforced in thematic-based practicals\, leading to 4-MC. \nMajor topics include:\ngene editingcellular structure and processesstatisticsbio-imagingimage analysis\,and quantitative methods in biologyThere will be extensive tutorials in using Python\, complemented by a hands-on microscopy and wet-lab sessions. Students will work as part of a team to tackle problems and to develop a report on an open area of modern bioscience research. \nThe biophysics of bacterial pathogenesis\nLike their larger multicellular hosts\, bacteria and other prokaryotic cells are subjected to\, and generate\, mechanical forces. When invading a host cell\, forces must be overcome\, and in some cases\, the dynamic structures of the host cell may be exploited by bacteria to facilitate their invasion. \nFor example\, bacteria can modulate the cell membrane and manipulate the cytoskeleton for entry into the host\, or for the secretion of virulence factors. In doing so the bacteria are able to establish an infection. However\, the precise molecular mechanisms underlying bacterial uptake\, and the mechanisms by which bacteria can evade the body’s defenses remain unclear. At MBI\, we are investigating the biophysical aspects of bacterial pathogenesis\, and in particular\, are interested in the mechanisms by which bacteria like Salmonella utilize the endocytic membrane trafficking pathway to survive within the host. In this case\, Salmonella will survive within an endosomal compartment called the salmonella containing vacuole (SCV). Salmonella also thrives within its host in a non-infectious phase for an extended period of time. Here\, the bacteria will exist as a biofilm. How salmonella lives within SCVs\, the various cytoskeletal modifications that take place during invasion and the regulatory mechanisms that are modulated to enable Salmonella to exist as a biofilm\, are all researched at MBI. \nAdditional work in this area is looking at the formation and nature of cytoskeletal pedestals\, which\, in the case Enteropathogenic E.coli (EPEC) infection\, are found within host cells at the site of bacterial attachment. \nCoordinators\n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \nLecturers\n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \nProgram\n \n\n Download Program Overviews\n \n \n \n \n\n Download Program Overviews\n \n \n \nHow to Register?\nClick this or go to the website www.registrationboothcamp.com . There is no registration fee for the conference. \nDeadline \n20th June 2021 \nRegistrants interested in submitting an abstract for an oral presentation (15 mins) may submit the abstract (200 word limit) via online registration. \nLocation \nLT7\, Engineering Block EA Building\, Level 3National University of Singapore5A Engineering Drive 1Singapore 117411 \n+65 6872 6123 \nCorporate Sponsors\n \n \n \nSupported By\n \n \nHow to Become Sponsor?\nMBI 3M 2021 presents an opportunity for sponsors to showcase technologies and build partnerships with researchers designing cutting-edge engineering tools for mechanobiology and other life science research. \nCurrently\, MBI 3M 2021 has 17 Satellite Centres across the Asia-Pacific\, providing sponsors with the ability to target this growing regional scientific community. As many of our Satellite Centres are also run by early-career researchers\, we expect our audience to be particularly interested in learning about the latest lab equipment\, bioengineering technology advances\, and publication venues that can assist their scientific investigations. \nIf you are interested in exploring sponsorship opportunities with MBI 3M 2021\, please contact us at mbi3mconference21@gmail.com. \nDeadline \n20th June 2021 \nRegistrants interested in submitting an abstract for an oral presentation (15 mins) may submit the abstract (200 word limit) via online registration. \nLocation \nLT7\, Engineering Block EA Building\, Level 3National University of Singapore5A Engineering Drive 1Singapore 117411 \n+65 6872 6123 \nThis ten-day “Bootcamp” style course will cover fundamental concepts and practical approaches in understanding cellular functions. The focus is to develop a breadth of knowledge in quantitative techniques to allow bioscience researchers to pursue further depth in their respective research work. \nThe Bootcamp lectures are open to all interested graduate students and post-docs. For graduate students the lectures will be reinforced in thematic-based practicals\, leading to 4-MC. \nMajor topics include:\ngene editingcellular structure and processesstatisticsbio-imagingimage analysis\,and quantitative methods in biologyThere will be extensive tutorials in using Python\, complemented by a hands-on microscopy and wet-lab sessions. Students will work as part of a team to tackle problems and to develop a report on an open area of modern bioscience research. \nThe biophysics of bacterial pathogenesis\nLike their larger multicellular hosts\, bacteria and other prokaryotic cells are subjected to\, and generate\, mechanical forces. When invading a host cell\, forces must be overcome\, and in some cases\, the dynamic structures of the host cell may be exploited by bacteria to facilitate their invasion. \nFor example\, bacteria can modulate the cell membrane and manipulate the cytoskeleton for entry into the host\, or for the secretion of virulence factors. In doing so the bacteria are able to establish an infection. However\, the precise molecular mechanisms underlying bacterial uptake\, and the mechanisms by which bacteria can evade the body’s defenses remain unclear. At MBI\, we are investigating the biophysical aspects of bacterial pathogenesis\, and in particular\, are interested in the mechanisms by which bacteria like Salmonella utilize the endocytic membrane trafficking pathway to survive within the host. In this case\, Salmonella will survive within an endosomal compartment called the salmonella containing vacuole (SCV). Salmonella also thrives within its host in a non-infectious phase for an extended period of time. Here\, the bacteria will exist as a biofilm. How salmonella lives within SCVs\, the various cytoskeletal modifications that take place during invasion and the regulatory mechanisms that are modulated to enable Salmonella to exist as a biofilm\, are all researched at MBI. \nAdditional work in this area is looking at the formation and nature of cytoskeletal pedestals\, which\, in the case Enteropathogenic E.coli (EPEC) infection\, are found within host cells at the site of bacterial attachment. \nCoordinators\n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \nLecturers\n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \n \n \nPakorn Tony KANCHANAWONG\nSuper-resolution microscopy\, integrin-mediated cell adhesions\, nanoscale architecture of cellular structures \nProgram\n \n\n Download Program Overviews\n \n \n \n \n\n Download Program Overviews\n \n \n \nHow to Register?\nClick this or go to the website www.registrationboothcamp.com . There is no registration fee for the conference. \nDeadline \n20th June 2021 \nRegistrants interested in submitting an abstract for an oral presentation (15 mins) may submit the abstract (200 word limit) via online registration. \nLocation \nLT7\, Engineering Block EA Building\, Level 3National University of Singapore5A Engineering Drive 1Singapore 117411 \n+65 6872 6123 \nCorporate Sponsors\n \nSupported By\n \nHow to Become Sponsor?\nMBI 3M 2021 presents an opportunity for sponsors to showcase technologies and build partnerships with researchers designing cutting-edge engineering tools for mechanobiology and other life science research. \nCurrently\, MBI 3M 2021 has 17 Satellite Centres across the Asia-Pacific\, providing sponsors with the ability to target this growing regional scientific community. As many of our Satellite Centres are also run by early-career researchers\, we expect our audience to be particularly interested in learning about the latest lab equipment\, bioengineering technology advances\, and publication venues that can assist their scientific investigations. \nIf you are interested in exploring sponsorship opportunities with MBI 3M 2021\, please contact us at mbi3mconference21@gmail.com. \nDeadline \n20th June 2021 \nRegistrants interested in submitting an abstract for an oral presentation (15 mins) may submit the abstract (200 word limit) via online registration. \nLocation \nLT7\, Engineering Block EA Building\, Level 3National University of Singapore5A Engineering Drive 1Singapore 117411 \n+65 6872 6123 URL:https://mbi.closelycoded.site/event/bootcamp-2021/ END:VEVENT END:VCALENDAR